Journal: Scientific Reports
Article Title: Correlation of BUB1 and BUB1B with the development and prognosis of endometrial cancer
doi: 10.1038/s41598-024-67528-2
Figure Lengend Snippet: Influence of BUB1 and BUBIB knockdown on EC cell phenotype. ( A ) The growth of Ishikawa cells in each group; ( B ) The migration rates of Ishikawa cells in each group: blank group (50.99 ± 1.02)%, NC-siRNA group (66.50 ± 7.74)%, BUB1-siRNA group (34.87 ± 4.26)%, and BUB1B-siRNA group (31.96 ± 4.72)%; ( C ) The number of cells that crossed the membrane in each group: blank group (127.3 ± 4.8), NC-siRNA group (125.3 ± 3.9), BUB1-siRNA group (48.6 ± 3.2), and BUB1B-siRNA group (35.0 ± 2.1). * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: Samples were incubated with primary antibody (rabbit anti-human BUB1 and BUBIB monoclonal antibody, 1:200) (Sanying Biotechnology Co. Ltd., Wuhan, Hubei, China), followed by incubation with secondary antibody (HRP-labeled goat anti-rabbit II antibody, 1:200) (Zhongshan Jinqiao Biotechnology Co. Ltd., Beijing, China).
Techniques: Knockdown, Migration, Membrane